Figure 1: Control plate containing BL21 E. coli and Ampicillin only, no plasmid pGEM-gbr22. Bacterial growth after incubation at 37 degrees Celsius overnight minimal, with only condensation present.
Figure 2: Experimental plate containing BL21 E. coli, Ampicillin, and plasmid pGEM-gbr22. Bacterial growth after incubation at 37 degrees Celsius overnight with multiple colonies scattered throughout.
Figure 3: Fun plate containing bacteria swabbed from trash cans, water fountain, doors, shoes, ground, and sink faucet. Bacterial growth after incubation at 37 degrees Celsius overnight with large colonies of bacteria and some spores.
Figure 4: Two Erlenmeyer flasks containing a large culture of BL21 E. coli with pGEM-gbr22. Bacteria were successfully transformed as evident through the characteristic purple color of the solution.
Figure 5: BL21 E. coli wet pellet derived from centrifuging the bacterial solution and disposing the supernatant liquid. Contained in a 50mL conical tube with a weight of 0.46 grams.
Figure 6: Elutions 1 and 2 contained in conical tubes. Derived from column filtration with nickel.
Figure 7: Cellophane wrapped, dry gel after drying for two hours. Lanes from bottom to top: ladder, cell lysate, soluble fraction, flow through, wash, Elution 1(Sri), Elution 2(Sri), Elution 1(Gio), and Elution 2(Gio).
Introduction:
Materials & Methods:
Results:
Discussion:
Conclusions:
References:
Figure 1: Control plate containing BL21 E. coli and Ampicillin only, no plasmid pGEM-gbr22. Bacterial growth after incubation at 37 degrees Celsius overnight minimal, with only condensation present.
Figure 2: Experimental plate containing BL21 E. coli, Ampicillin, and plasmid pGEM-gbr22. Bacterial growth after incubation at 37 degrees Celsius overnight with multiple colonies scattered throughout.
Figure 3: Fun plate containing bacteria swabbed from trash cans, water fountain, doors, shoes, ground, and sink faucet. Bacterial growth after incubation at 37 degrees Celsius overnight with large colonies of bacteria and some spores.
Figure 4: Two Erlenmeyer flasks containing a large culture of BL21 E. coli with pGEM-gbr22. Bacteria were successfully transformed as evident through the characteristic purple color of the solution.
Figure 5: BL21 E. coli wet pellet derived from centrifuging the bacterial solution and disposing the supernatant liquid. Contained in a 50mL conical tube with a weight of 0.46 grams.
Figure 6: Elutions 1 and 2 contained in conical tubes. Derived from column filtration with nickel.
Figure 7: Cellophane wrapped, dry gel after drying for two hours. Lanes from bottom to top: ladder, cell lysate, soluble fraction, flow through, wash, Elution 1(Sri), Elution 2(Sri), Elution 1(Gio), and Elution 2(Gio).